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KMID : 0545120180280040527
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 4 p.527 ~ p.533
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Protection of Radiation-Induced DNA Damage by Functional Cosmeceutical Poly-Gamma-Glutamate
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Oh Yu-Jin
Kwak Mi-Sun
Sung Moon-Hee
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Abstract
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Poly-gamma-glutamate (γ-PGA), an anionic polymer of glutamic acid bonded by γ-amide linkages, is a very promising biodegradable polymer isolated from the Bacillus subtilis sp. Chungkookjang found in Korean fermented food. γ-PGA is useful in a wide range of industrial fields, such as food, cosmetics, and medicine as it is water-soluble, anionic, biodegradable, and edible. In this study, we compared the radioprotective effects of high-molecular-weight 3,000 kDa γ-PGA and reduced form of glutathione (GSH known as radioprotector) on DNA damage. Radiation-induced DNA damage was measured by decrease of fluorescence intensity after DNA bind with ethidium bromide (EtBr). All experiments were performed 60Co gamma (γ) radiation at 1252 Gy with 50% DNA damage. As the concentration of 0.33 μM-1.65 μM γ-PGA increased, DNA protection from radiation-induced damage also increased, with a maximum of 87% protection. On the other hand, DNA protection of GSH with increasing concentration was up to maximally 70%. In this study, we demonstrate that γ-PGA exhibited significant radioprotective effects against gamma irradiation.
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KEYWORD
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Poly-gamma-glutamate, radioprotective material, gamma radiation, DNA damage, DNA protection
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